PHYTOCHEMICAL AND PHARMACOLOGICAL STUDIES OF THE LEAVES OF OLAX MANN1 OLIV [OLACACEAE]

PHYTOCHEMICAL AND PHARMACOLOGICAL STUDIES OF THE LEAVES OF OLAX MANN1 OLIV [OLACACEAE]

Abstract:

The Plant Olax manni. is a shrub widely distributed in West Africa and it is employed in traditional medicine in Northern Nigeria to treat poisonous snakebites. General phytochemical examination of the various crude extracts of the leaves of this plant revealed the presence of tannins, fatty acids, saponins, sterioids/terpenoids. Compound C1 was isolated from (he petroleum ether extract of the leaves. This was shown to be 5 (0-methoxy phenyl) pent – 2,4 – dienoic aeid using spectroscopic studies (UV, IR, HNMR, 13C-NMR, COSY and DEPT). Invivo anti-snake venom studies of the methanolic extract against venom of Naja nigricollis and Echis carinatus using Swiss albino mice showed significant anti venin activity. The extract was shown to possess protective activity against the venom of these two poisonous snakes. However, the anti-venin activity is more pronounced against the venom of N. nigricollis.

TABLE OF CONTENT
Title page- – – – – – – – – i
Approval page – – – – – – – -ii
Dedication – – – – – – – – -iii
Acknowledgement – – – – – – – -iv
Abstract – – – – – – – – – -v
Table of content – – – – – – – -vi

CHAPTER ONE
INTRODUCTION – – – – – – – -1
1.0 Background of the study – – – – -1
1.1 Statement of the problem – – – – -5
1.2 Purpose of the study – – – – – -6
1.3 Significance of the study – – – – -8
1.4 Research questions – – – – – -9
1.5 Scope of the study – – – – – – -10

CHAPTER TWO

LITERATURE REVIEW – – – – – – -11

CHAPTER THREE

Research methodology – – – – – – -39
Design of study – – – – – – – -40
Area of study – – – – – – – – -40
Population of the study – – – – – – -41
Sample and sampling techniques – – – – -41
Instrument for data collection – – – – -41
Method of data collection – – – – – -42
Method of data analysis – – – – – – -43

CHAPTER FOUR

Presentation, analysis and interpretation of data – -48
Discussion of findings – – – – – – -56

CHAPTER FIVE

Summary of findings – – – – – – -60
Conclusion – – – – – – – – -61
Recommendations – – – – – – – -62
Suggestions for further research – – – – -64
References – – – – – – – – -65
Appendix I – – – – — – – – -68
Questionnaire. – – – – – – – -69

PHYTOCHEMICAL AND PHARMACOLOGICAL STUDIES OF THE LEAVES OF OLAX MANN1 OLIV [OLACACEAE]