PHYTOCHEMICAL AND ANTIMICROBIAL STUDIES OF Spermacoce verticillata (Rubiaceae) G.F.W. Meyer

PHYTOCHEMICAL AND ANTIMICROBIAL STUDIES OF Spermacoce verticillata (Rubiaceae) G.F.W. Meyer

Abstract:

The plant Spermacoce verticillata is used in ethnomedicine for the treatment of itches, diarrhoea, sores and other ailments. The hexane soluble fraction of acetone extract of the whole plant Spermacoce verticillata was subjected to phytochemical and antimicrobial screening. The phytochemical techniques employed were dry column vacuum chromatography and preparative thin layer chromatography. The antimicrobial activity was studied using agar diffusion, and broth dilution methods. The result of preliminary phytochemical screening carried out on the crude acetone extract revealed the presence of carbohydrates, Flavonoids, Anthraquinones, terpernoids, Tannis and Steroids; Tannins flavonoids, and Steroids/terpenes were also found to be present in the hexane fraction. Extensive chromatographic separation of the hexane soluble fraction using silica gel, dry column chromatography, followed by preparative thin layer chromatography led to the isolation of stigmasterol. The structure of this compound was established by spectral analysis including 1D and 2D NMR. The result suggest that the hexane fraction of Spermacoce verticillata plant possess phytochemical constituents that may be useful in the management of microbial diseases.

PHYTOCHEMICAL AND ANTIMICROBIAL STUDIES OF Spermacoce verticillata (Rubiaceae) G.F.W. Meyer