EVALUATING THE EFFECT OF MOMORDICA BALSAMINA LINN, SELENIUM AND LAMIVUDINE FOR THE TREATMENT OF NEWCASTLE DISEASE IN PULLETS

EVALUATING THE EFFECT OF MOMORDICA BALSAMINA LINN, SELENIUM AND LAMIVUDINE FOR THE TREATMENT OF NEWCASTLE DISEASE IN PULLETS

Abstract:

Newcastle disease virus is an avian paramyxovirus that causes significant economic losses to the poultry industry worldwide. Aim of the study was to evaluate the effect of M. balsamina, selenium,lamivudine and nutritional substances while the objectives of the study were to : Determine the phytochemical of M. balsamina, determine the nutritional substances of M. balsamina, determine the median lethal dose of M. balsamina in pullets and evaluate the effect of M. balsamina, selenium and lamivudine weather they can be used in the treatment of Newcastle disease. A total of three hundred day-old dominant black pullets obtained from a commercial hatchery in Ibadan Nigeria were randomly assigned into six groups. Group 1 was pretreated orally with 300 mg of aqueous extract of Momordica balsamina per bird for 1 week before challenge with Newcastle disease virus (NDV) Kudu 113. Birds in group II were inoculated with NDV Kudu 113 and then treated immediately with 300 mg of extract of M. balsamina for 2 weeks. Birds in group III were inoculated with NDV Kudu 113 and immediately treated with lamivudine (300 mg /litre) of water per 40 birds for 2 weeks/40 birds. Group IV was inoculated with NDV Kudu 113 and treated immediately with selenium 1 ml/litre of water and group V and VI were negative and positive controls respectively. All negative and positive control groups were treated with drinking water for 2 weeks orally. Birds in week V were given 25 ml/ bird for 2 weeks. Birds were bled on weekly basis on day- old, 4th week, 5th week, 6th week and 7th week. Serum was obtained by allowing the blood collected from each bird to stand for 3 hours at room temperature. Proximate composition of M. balsamina was performed at a temperature of 500-600 Ċ. The Phytochemical composition of M. balsamina fruit showed that it contains tannins (0.0008 % ), saponins (23.99 %) and alkaloids (6.94 %). Proximate composition of M. balsa mina indicated high amount of carbohydrate (27.07 %) compare to low crude protein, crude fibre (15.80 %) and ash (11.10 %). Clinical signs of ND recorded in groups due to Newcastle disease in pullets were : Group I inactive, emaciation, dollness, ruffle feather, anorexia, weakness, coughing, sneezing, greenish white diarrhea, salivation, mortality 5,9,8,1,7. Group II : Inactive, emaciation, dollness, ruffle

EVALUATING THE EFFECT OF MOMORDICA BALSAMINA LINN, SELENIUM AND LAMIVUDINE FOR THE TREATMENT OF NEWCASTLE DISEASE IN PULLETS