QUANTITATIVE DETERMINATION OF CARBAZOLE ALKALOIDS FROM CRUDE PLANT EXTRACT (Glycosmis pentaphylla of family Rutaceae) USING GAS LIQUID CHROMATOGRAPHY

QUANTITATIVE DETERMINATION OF CARBAZOLE ALKALOIDS FROM CRUDE PLANT EXTRACT (Glycosmis pentaphylla of family Rutaceae) USING GAS LIQUID CHROMATOGRAPHY

Abstract:

Quantitation of carbazole alkaloids (Carbazole, 3-Methyl Carbazole.Glycozoline and Glycozolidine) was carried out using Gas-Liquid Chromatography (GLC ). This was -the first time a quantitative work was undertaken on carbazole alkaloids© A stock solution of 1mg/ml in chloroform was prepared of each of the above mentioned carbazole alkaloids© Appropriate working concentration were prepared by suitable dilutions of various stock solutions .with chloroform. Heptazolidine , (lmg/ml) in chloroform, was used as the internal standard (i.s.) The plant extract (Glycosmis Pentaphylla of family Rutaceas) solution (unknown sample) was prepared by drawing a drop (weighed 23mg) from the light Petroleum extract by using a clean glass rod and diluting it to 5ml with chloroform. The I.S. (1ml) was added to all solutions prior to analysis© All samples (3ul) were chromatographed on a Bye Unicam series 204 chromatograph equipped with FID and Temperature programmer© A single point internal standard method was employed for the analysis of result. TLG was performed of carbazole, 3 – methyl carbazole and the Crude plant extract© Single point method employed was faster and more convenient that the traditional individual calibration curve method. The concentrations of the alkaloids were found as:- 1. Carbazole – 17.35 ug/ml (0.4% w/w) 2. 3-Methyl Carbazole – 186.67 • (4.06% * ) 4. Glycozolidine – 189.70 ” (4.1 2% ” ) plant TLC results added supportive evidence that the/extract contains carbazole and 3 – methyl-carbazole (all identified for the first time from this plant species)c G-LC was found to be a very convenient means of finding the levels of components in a plant extract.

QUANTITATIVE DETERMINATION OF CARBAZOLE ALKALOIDS FROM CRUDE PLANT EXTRACT (Glycosmis pentaphylla of family Rutaceae) USING GAS LIQUID CHROMATOGRAPHY